sp17  (Proteintech)

Journal: Frontiers in Immunology

Article Title: Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis

doi: 10.3389/fimmu.2022.844736

Figure Lengend Snippet: Basic information of SPA17. (A) The level of SPA17 expression between tumor and normal tissues in each type of cancer based on the integrated data from TCGA and GTEx datasets. (B) Analysis of SPA17 change frequency in pan-cancer research according to cBioPortal database. (C) The SPA17 expression levels between SPA17-deletion and diploid TGCT patients. (D) The immunofluorescence images of SPA17 protein, nucleus, endoplasmic reticulum (ER), microtubules and the incorporative images in A-431 and U251 cell lines. (E) The protein-protein interaction (PPI) network presents the proteins interacting with SPA17. (F, G) Western blot protein detection of the SPA17 expression levels in paired GBM and adjacent normal tissues. The labelled asterisk indicated the statistical p-value (ns, p > 0.05, *p < 0.05, **p < 0.01 and ***p < 0.001).

Article Snippet: The primary SPA17 and beta-tubulin rabbit polyclonal antibodies were obtained from the Proteintech company (13367-1-AP, 10094-1-AP), and the concentrations of antibodies were entirely following their specifications.

Techniques: Expressing, Immunofluorescence, Western Blot

Journal: Frontiers in Immunology

Article Title: Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis

doi: 10.3389/fimmu.2022.844736

Figure Lengend Snippet: (A) Based on the univariate Cox regression and Kaplan-Meier models, summary of the correlation between SPA17 expression and overall survival (OS), disease-specific survival (DSS), disease-free interval (DFI) and progression-free interval (PFI). Red indicates that SPA17 is a risk factor for the prognosis of cancer patients, while blue represents a protective factor. Only p-values < 0.05 are shown. (B) The forest plot showed the prognostic role of SPA17 in cancers by univariate Cox regression. The red type of cancer represents SPA17 as a statistically significant risk factor, and blue represents a protective factor. (C–F) Kaplan-Meier overall survival curves of SPA17 in BRCA (C) , KIRC (D) , LGG (E) and TGCT (F) .

Article Snippet: The primary SPA17 and beta-tubulin rabbit polyclonal antibodies were obtained from the Proteintech company (13367-1-AP, 10094-1-AP), and the concentrations of antibodies were entirely following their specifications.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis

doi: 10.3389/fimmu.2022.844736

Figure Lengend Snippet: The hallmarks gene set enrichment analysis (GSEA) of SPA17 in pan-cancer. The size of the circle represents the false discovery rate (FDR) value of each cancer enrichment item, and the color represents the normalized enrichment score (NES) of each enrichment item.

Article Snippet: The primary SPA17 and beta-tubulin rabbit polyclonal antibodies were obtained from the Proteintech company (13367-1-AP, 10094-1-AP), and the concentrations of antibodies were entirely following their specifications.

Techniques:

Journal: Frontiers in Immunology

Article Title: Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis

doi: 10.3389/fimmu.2022.844736

Figure Lengend Snippet: The correlations of SPA17 expression and the infiltration levels of CD4+ T cells, CAF, progenitor, Endo, Eos, HSC, Tfh, γδT, NKT, regulatory T cells (Tregs), B cells, neutrophils, monocytes, macrophages, dendritic cells, NK cells, Mast cells and CD8+ T cells in cancers. Positive correlation in red and negative correlation in blue.

Article Snippet: The primary SPA17 and beta-tubulin rabbit polyclonal antibodies were obtained from the Proteintech company (13367-1-AP, 10094-1-AP), and the concentrations of antibodies were entirely following their specifications.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis

doi: 10.3389/fimmu.2022.844736

Figure Lengend Snippet: (A) The Spearman correlation heatmap shows the correlation between the expression of SPA17 and 47 kinds of immune regulators in pan-cancer. Red represents positive correlation and blue represents negative correlation. (B) Correlations between SPA17 expression and tumor mutation burden in pan-cancer. (C) Correlations between SPA17 expression and microsatellite instability in pan-cancer. (D) Kaplan-Meier curve of low and high-SPA17 subgroup in IMvigor210 cohort (anti-PD-L1), and the proportion of tumors (including kidney cancer) in the low-SPA17 and high-SPA17 subgroups in the IMvigor210 cohort who responded to PD-1 therapy. (E) Kaplan-Meier curve of GSE91061 (anti-PD-L1 melanoma) in the low and high-SPA17 patient groups, and the proportion of melanoma patients in the GSE91061 low and high-SPA17 subgroups that responded to anti-PD-1 therapy. The labelled asterisk indicated the statistical p-value (*p < 0.05, **p < 0.01, ***p < 0.001).

Article Snippet: The primary SPA17 and beta-tubulin rabbit polyclonal antibodies were obtained from the Proteintech company (13367-1-AP, 10094-1-AP), and the concentrations of antibodies were entirely following their specifications.

Techniques: Expressing, Mutagenesis

Journal: BMC cancer

Article Title: Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study.

doi: 10.1186/s12885-018-4880-x

Figure Lengend Snippet: Fig. 1 Representative Sp17 immunohistochemistry staining in ovarian tissue of serous histology. Examples of Sp17 protein expression in benign and malignant ovarian tissue demonstrating both positive and negative staining and intensity of Sp17 staining are shown. a) Serous cystadenoma showing tissue staining positive with strong expression of Sp17 in ciliated cells. b) Serous borderline tumor showing strong Sp17 expression. c) Grade 1 serous adenocarcinoma negative for Sp17 expression. d) Grade 1 serous adenocarcinoma showing strong Sp17 expression. e) Grade 3 serous adenocarcinoma negative for Sp17. f) Grade 3 serous adenocarcinoma demonstrating strong Sp17 staining. Stroma surrounding epithelial cells is negative

Article Snippet: The TMA or whole mount slides were incubated with affinity purified rabbit polyclonal antibody to Sp17 (Proteintech, Rosemont, IL, USA) used at a 1:300 dilution following standard deparaffinization in xylene and heat-induced antigen retrieval in citrate buffer, pH 6.0.

Techniques: Immunohistochemistry, Staining, Expressing, Negative Staining

Journal: BMC cancer

Article Title: Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study.

doi: 10.1186/s12885-018-4880-x

Figure Lengend Snippet: Fig. 2 Sp17 RNA expression is upregulated in borderline ovarian cancers. Sp17 mRNA expression was analyzed from two publically available patient tumor Oncomine datasets. a Sp17 mRNA levels are significantly increased in borderline ovarian serous adenocarcinoma when compared to ovarian serous adenocarcinoma (p < 0.001) in Anglesio et al. dataset [18]. b Sp17 mRNA levels are significantly increased in borderline ovarian serous adenocarcinoma when compared to ovarian adenocarcinoma (p < 0.001) in Tothill et al. dataset [17] (c) No significant difference in Sp17 expression is seen between grades of ovarian adenocarcinoma (p = 0.42) [17]. Box encompasses 25th–75th percentile, solid black line indicates median and error bars indicate range. Numbers in parentheses indicate number of cases included in the analysis

Article Snippet: The TMA or whole mount slides were incubated with affinity purified rabbit polyclonal antibody to Sp17 (Proteintech, Rosemont, IL, USA) used at a 1:300 dilution following standard deparaffinization in xylene and heat-induced antigen retrieval in citrate buffer, pH 6.0.

Techniques: RNA Expression, Expressing

Journal: BMC cancer

Article Title: Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study.

doi: 10.1186/s12885-018-4880-x

Figure Lengend Snippet: Fig. 3 Serum Sp17 concentration compared to histology and tissue expression. a Distribution of serum Sp17 concentration in benign ovarian neoplasms, borderline ovarian tumors (BOTs), and epithelial ovarian carcinomas (EOCs). The serum concentration of Sp17 is plotted in standard box and whisker plots on a log 10 scale by histology. No significant difference was found between the serum Sp17 concentration when comparing benign ovarian neoplasms, BOTs, and EOCs. ANOVA test showed that there was a significant difference (p < 0.001) in serum Sp17 concentration among the four main histologic subtypes of EOC: serous, mucinous, endometrioid, and clear cell. Box (interquartile range) encompasses 25th–75th percentile, solid black line indicates median and the whiskers indicate the lowers and highest data values that are still within the 25th and 75th percentile value plus 1.5 times the interquartile range, respectively. The outliers are represented as separate data points. Numbers in parentheses indicate number of cases included in the analysis. b Distribution of serum Sp17 concentration by tissue expression of Sp17. There were 65 patients with both serum and ovarian tissue available, which included benign, borderline, and malignant ovarian neoplasms. The ovarian tissue underwent immunohistochemical (IHC) staining for Sp17 and any expression was considered a positive result. The matching sera for these patients were analyzed for Sp17 concentration using ELISA and the concentration converted to a log 10 scale and represented here in box and whisker plots. There was a significant difference in serum Sp17 levels between IHC positive neoplasms compared to IHC negative neoplasms (p = 0.027), with IHC positive neoplasms showing higher SP17 levels. Box (interquartile range) encompasses 25th–75th percentile, solid black line indicates median and the whiskers indicate the range. Numbers in parentheses indicate number of cases included in the analysis

Article Snippet: The TMA or whole mount slides were incubated with affinity purified rabbit polyclonal antibody to Sp17 (Proteintech, Rosemont, IL, USA) used at a 1:300 dilution following standard deparaffinization in xylene and heat-induced antigen retrieval in citrate buffer, pH 6.0.

Techniques: Concentration Assay, Expressing, Whisker Assay, Immunohistochemical staining, Immunohistochemistry, Enzyme-linked Immunosorbent Assay